Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to <i>Botrytis cinerea</i> and Reactive Oxygen Species

Plants require interaction between signaling pathways to differentiate and integrate stress responses deploy appropriate defenses. The hormones ethylene, salicylic acid (SA), jasmonic (JA) are important regulators of plant Numerous interactions these the cornerstone robust immunity. Additionally, during early response pathogens, reactive oxygen species (ROS) act as molecules. Here, we examined extent signal in stages Botrytis cinerea infection. To enable a comparison B. infection with ROS signaling, subjected plants ozone treatment, which stimulates an apoplastic burst. We used collection single, double, triple mutants defective hormone biosynthesis them treatment at different timepoints. lesion size, cell death, gene expression (both quantitatively spatially). two treatments shared many similarities, especially JA-insensitive mutants, were sensitive both treatments. Unexpectedly, cinerea–susceptible mutant (coi1), became tolerant when SA was impaired (coi1 npr1 sid2), demonstrating that JA may be under control SA. Extensive marker analysis indicated main regulator In addition, identified transcription factor SR1 crucial PLANT DEFENSIN cell-death regulation, contributes resistance cinerea. Overall, our work further defines context defense signaling. [Formula: see text] Copyright © 2021 Author(s). This is open access article distributed CC BY-NC-ND 4.0 International license .